猪干扰素调节因子7的克隆及原核表达

doi:10.11843/j.issn.0366-6964.2014.08.002

Abstract

Abstract:

To gain further insight into the biological functions of IRF7 gene，the open reading frame of the target gene was amplified from the cDNA of porcine duodenum，and cloned into the prokaryotic expression vector pET-21b(+) to generate a pET-21b-IRF7 recombinant plasmid.After verification by sequencing，the pET-21b-IRF7 was constructed，and then transformed into E.coli BL21(DE3).The His-IRF7 fusion protein was induced by IPTG and then identified by Western blot.The results shown that：(1) The open reading frame of 1 461 bp was successfully cloned.(2)His-IRF7 fusion protein was efficiently expressed in the lysate in the form of both soluble cysteine and inclusion bodies,with a molecular weight of about 60.5 ku.These results indicated that IRF7 fusion protein was successfully expressed in E.coli BL21(DE3)，which lays the foundation for the related function studies of porcine IRF7.

CLC Number:

S828
S813.3

ZHANG Xue-mei，DU Li-li，LIU Fu-tao，WANG Jiang，GUO Yu-jie，LI Hong-ji，YANG Guo-yu. Cloning and Prokaryotic Expression of the Porcine Interferon Regulatory Factor 7 (IRF7)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1213-1217.

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Cloning and Prokaryotic Expression of the Porcine Interferon Regulatory Factor 7 (IRF7)

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